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- From: Colin Poczatek <jpoczatek@rics.bwh.harvard.edu>
- To: iv3d-users@sci.utah.edu
- Cc: Maxence Le Vasseur <maxencelevasseur@hotmail.com>
- Subject: [IV3D-USERS] Re: Re: ImageVis3D
- Date: Tue, 30 Nov 2010 13:07:12 -0500
FYI there's a nrrd plugin for ImageJ that can handle single channel
nrrd's. FWIW we use a modified version in our ImageJ plugin to
save/open 3D + channel nrrd's.
http://flybrain.stanford.edu/nrrd
Collin
tom fogal wrote:
>
Hi Max,
>
>
Maxence Le Vasseur <maxencelevasseur@hotmail.com> writes:
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>
> I converted my images in TIFF and created a Z-stack of tiff images
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> (in a single file).
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>
>
>
Oooh, ouch. Due to an impedance mismatch between how TIFF stores 3D
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data and how we store 3D data, 3D TIFFs are probably the worst format
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to put your data in, because accessing it is so slow. See the "Getting
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Data Into ImageVis3D" manual for detailed information:
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>
http://ci.sci.utah.edu:8011/devbuilds/GettingDataIntoImageVis3D.pdf
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>
As a quick fix: a series of 2D TIFFs, and then loading from a directory
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instead of a file would probably work better.
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>
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> I was able to open and convert some stacks using ImageVis3D but now
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> it seems impossible to do so. Everytime I try to convert a stack the
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> software crashes for no apparent reason.
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>
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> I was wondering if someone in your team had faced a similar problem
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> in the past and if you had found a way around it.
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>
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The 3D TIFF converter was written for a specific collaboration, and I
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experienced a lot of difficulty finding sample data on the web. Thus
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I'm not surprised if there are lurking issues; can't fix what I can't
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reproduce :)
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I'd very much like to fix said lurking issues, though. Could you use
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the "Report an Issue" feature (under Help) and attach one of the TIFFs
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which is failing to load, please?
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>
As a workaround to get you going ASAP, I'd bet the series-of-2D-TIFFs
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is your easiest route, since you're already familiar with TIFFs. The
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QVis, NRRD, BOV, and (of course) raw formats can be pretty easy too.
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>
Thanks,
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>
-tom
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